Abstract
Platelet is one of the cells within the blood that have potential in regenerative therapy. Recently platelet-related products got special attention due to the abundance of growth factors and easy availability and processing. With the robust in cell-based therapy, platelet-rich plasma (PRP) has been intensively studied for its potential to substitute the fetal bovine serum (FBS) as the supplement in cell culture. Our study aimed to investigate the use of human PRP in mesenchymal stem cells (MSC) culture and its related effect on stem cell biology. We searched in vitro studies that used human PRP as a supplementing factor on human MSCs culture. From the initial 172 studies, 14 studies fulfilled the selection criteria and were analysed. The results showed that the sources of MSCs were varied, including adipose tissue, bone marrow, and dental tissue. The PRP concentration showing the best effects was ranged from 10 to 20%. In addition, most of the studies demonstrated the superiority of PRP to FBS in promoting the proliferation and differentiation of MSCs in vitro. Therefore, PRP could be an alternative to FBS in supporting a xeno-free culture system. Studies are needed to reveal the mechanism of PRP in maintaining the physiology of MSCs.
Highlights
To date, successes in regenerative medicine have included regenerating non-functional tissue or organ damage caused by trauma, congenital abnormalities, and some end-stage diseases [1,2]
Based on the selection methods, 172 articles were initially found (Fig. 1). Most of these articles reflect the use of platelet-rich plasma (PRP) in stem cell experiments, it is evident that the use of PRP as an alternative to animal sera is still not widely implemented
The articles showed PRP application in related to mesenchymal stem cells (MSC) in various methods; these included in vivo study designs using animals, which were used conjointly to scaffold or by directly applying the PRP alone or with the stem cell for tissue repair or regeneration without involving prior cell culture
Summary
Successes in regenerative medicine have included regenerating non-functional tissue or organ damage caused by trauma, congenital (acquired) abnormalities, and some end-stage diseases [1,2]. Mesenchymal stem cells (MSCs) are among the promising stem cells for cell-based therapy. MSCs are known to have the pluripotent ability to differentiate into several cell types, including adipocytes, osteoblasts, and chondrocytes [3]. To define the population of MSCs, there are minimal criteria according to The International Society for Cellular Therapy (ISCT). The cells have the ability to adhere on the plastic culture dish when cultured in vitro. The cells positively express (>95%) the surface antigen of CD73, CD90 and CD105; but negatively (
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