Abstract
We had previously described the design of a human polymeric immunoglobulin receptor mimetic peptidic ligand, pep14 (CITLISSEGYVSSK), that bound human IgM (hIgM) selectively and exhibited negligible affinity for other Ig proteins. In the present study, pep14 was investigated for its efficacy as an affinity ligand for IgM purification. Binding buffers at varying pH and ionic strengths were investigated. 10mM HEPES containing 150mM NaCl at pH 7.4 was found to be the optimum binding buffer. Equilibrium studies on pep14-immobilized-silica microspheres indicated a binding capacity of 5.9mg hIgM/g of silica–amine microspheres and an association constant of 3.2×106M−1. Exposure of pep14 to a synthetic mixture comprising hIgM, hIgG1 and bovine serum albumin had no adverse effect on the binding capacity and specificity of pep14. Experimental results highlighted the uniqueness of pep14 in capturing hIgM even at concentrations as low as 10μg/mL. Surface plasmon resonance based studies suggested that while pep14 exhibited affinity to human and rabbit IgM, pep14 failed to interact with mouse IgM. pep14 was resistant to column-sanitizing agents including 20% ethanol and 70% ethanol, although exposure to 100mM and 500mM sodium hydroxide resulted in hydrolysis.
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