Human Peripheral Blood Dendritic Cell and T Cell Activation by Codium fragile Polysaccharide.

Wei Zhang,Jihoe Kim,Juyoung Hwang,Sangguan You,Hae-Bin Park,Seulgi Go,Jun-O Jin,Inho Choi,Seong-Min Lim

doi:10.3390/md18110535

Abstract

Natural polysaccharides exhibit an immunostimulatory effect with low toxicity in humans and animals. It has shown that polysaccharide extracted from Codium fragile (CFP) induces anti-cancer immunity by dendritic cell (DC) activation, while the effect of CFP has not examined in the human immune cells. In this study, we found that CFP promoted the upregulation of CD80, CD83 and CD86 and major histocompatibility complex (MHC) class I and II in human monocyte-derived dendritic cells (MDDCs). In addition, CFP induced the production of proinflammatory cytokines in MDDCs. Moreover, CFP directly induced the activation of Blood Dendritic Cell Antigen (BDCA)1+ and BDCA3+ subsets of human peripheral blood DCs (PBDCs). The CFP-stimulated BDCA1+ PBDCs further promoted activation and proliferation of syngeneic CD4 T cells. The CFP-activated BDCA3+ PBDCs activated syngeneic CD8 T cells, which produced cytotoxic mediators, namely, cytotoxic T lymphocytes. These results suggest that CFP may be a candidate molecule for enhancing immune activation in humans.

Highlights

Marine natural products are promising sources of biologically active molecules [1,2].Various biological modulating compounds have been extracted from marine organisms, such as sponges, algae, and mollusks [3,4]
We evaluated the effect of C. fragile polysaccharide (CFP) in the activation of human peripheral blood dendritic cells (DCs) (PBDCs)
The results suggest that CFP can activate human PBDC subsets

Summary

Introduction

Marine natural products are promising sources of biologically active molecules [1,2]. Various biological modulating compounds have been extracted from marine organisms, such as sponges, algae, and mollusks [3,4]. In the field of immune modulation, there are a number of well-studied algae-derived polysaccharides, such as fucoidan, ascophyllan, carrageenan, and porphyran [5,6,7,8,9,10]. In previous studies it was found that fucoidan (extracted from brown algae) promoted the activation of dendritic cells (DCs), T cells and natural killer (NK) cells, which further induced anti-cancer immunity [10]. Polysaccharides extracted from brown algae have been well-studied with respect to immune activation in humans and mice, whereas green algae-extracted polysaccharides have not

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Conclusion