HUMAN PAPILLOMAVIRUS INFECTION GENOTYPE IN INFERTILE WOMEN AND ITS IMPACT ON REPRODUCTIVE OUTCOMES OF INTRAUTERINE INSEMINATION

Abstract

The purpose of this study was to evaluate the prevalence, genotype of HPV infection in women attending assistant reproductive treatment and to assess the relationship between HPV infection and outcome of intrauterine insemination (IUI). A observational study in a tertiary hospital in southern China During December 2019 and July 2019, cervical-swab samples were collected for detecting HPV from 1743 infertile women seeking for assistance at the assisted reproductive technology center (ART). 28 HPV genotypes were detected, including 18 high-risk HPV (hrHPV) genotypes (types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73 and 82), and 10 low-risk HPV (lrHPV) genotypes (types 6, 11, 40, 42, 43, 44, 54, 61, 81 and 83). We evaluated the prevalence of HPV in infertile women and evaluated associations between HPV and IUI reproductive outcome. 181 (10.38%) of the 1743 infertile women tested positive for HPV. Of these, 117 (64.64%) tested positive only for high-risk subtypes, 51 (28.18%) tested positive only for low-risk subtypes, and 13 (7.18%) tested positive for both high- and low-risk subtypes. Overall, 7.46% of all patients tested (130/1743) were positive for a high-risk type. The most common HrHPV genotype was HPV52 (2.29%), followed by HPV16 (1.03%), HPV58 (0.98%), HPV51 (0.80%). For the LrHPV genotypes, the most common type was HPV44 (1.15%), followed by HPV81 (0.57%), HPV42 (0.52%).The prevalence of HPV infection was higher in the age group of 41-50 years (14.94%) than in the age group of 21-30 years (9.33%) and 31-40 years (9.73%). For women undergoing IUI, the chemical pregnancy rate of hrHPV infection group (27.27%), lrHPV infection group (25.0%) were higher than non-HPV infection group (12.85%). 10.38% of the infertile women tested positive for HPV, of these 71.82% were positive for a high-risk type. The most common HPV genotype was HPV52 for HrHPV and HPV44 for the LrHPV. For those undergoing IUI, HPV infected women benefit more than non-HPV infected women.