Abstract
IntroductionPersistence of human papillomavirus (HPV) infections is associated with squamous cell carcinomas of different human anatomic sites. Several studies have suggested a potential role for HPV infection, particularly HPV16 genotype, in rectal cancer carcinogenesis.. The aim of this study was to assess the frequency of oncogenic HPV 16 viral DNA sequences in rectal carcinomas cases retrieved from the pathology archive of Braga Hospital, North Portuga.MethodsTaqMan-based type-specific real-time PCR for HPV 16 was performed using primers and probe targeting HPV16 E7 region.ResultsMost of the rectal cancer patients (88.5%, n = 206 patients), were symptomatic at diagnosis. The majority of the lesions (55.3%, n = 129) presented malignancies of polypoid/vegetant phenotype. 26.8% (n = 63) had synchronic metastasis at diagnosis. 26.2% (n = 61) patients had clinical indication for neoadjuvant therapy. Most patients with rectal cancer were stage IV (19.7% patients), followed by stage IIA (19.3%) and stage I (18.5%). All cases of the present series tested negative for HPV16.ConclusionThe total of negative tests for HPV 16 infection is a robust argument to support the assumption that HPV 16 infection, despite of previous evidences, is not involved in rectal cancer carcinogenesis and progression.
Highlights
Persistence of human papillomavirus (HPV) infections is associated with squamous cell carcinomas of different human anatomic sites
Despite of several studies have focused on the association of HPV and RC, few reports dedicate a large study on rectal cancer
Based on this lack of precise information about this topic, we sought to investigate the frequency of HPV 16, and its potential association with rectal carcinoma
Summary
Persistence of human papillomavirus (HPV) infections is associated with squamous cell carcinomas of different human anatomic sites. The aim of this study was to assess the frequency of oncogenic HPV 16 viral DNA sequences in rectal carcinomas cases retrieved from the pathology archive of Braga Hospital, North Portuga. Despite of several studies have focused on the association of HPV and RC, few reports dedicate a large study on rectal cancer, . Based on this lack of precise information about this topic, we sought to investigate the frequency of HPV 16, and its potential association with rectal carcinoma. The current study was designed to assess the incidence of HPV16 viral DNA sequences in rectal cancer carcinomas derived from a series of RC patients treated at Braga Hospital, North Portugal
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