Human papillomavirus genotyping and e6/e7 mRNA expression in greek women with intraepithelial neoplasia and squamous cell carcinoma of the vagina and vulva.

Elpida Tsimplaki,Issidora Papassideri,Maria Kouvousi,Lina Michala,Efstathia Panotopoulou,Elena Argyri,Aikaterini Apostolaki,George Magiakos

doi:10.1155/2012/893275

Elpida Tsimplaki, Issidora Papassideri + Show 6 more

Open Access

https://doi.org/10.1155/2012/893275

Copy DOI

Abstract

A large proportion of vaginal and vulvar squamous cell carcinomas (SCCs) and intraepithelial neoplasias (VAIN and VIN) are associated with HPV infection, mainly type 16. The purpose of this study was to identify HPV genotypes, as well as E6/E7 mRNA expression of high-risk HPVs (16, 18, 31, 33, and 45) in 56 histology samples of VAIN, VIN, vaginal, and vulvar SCCs. HPV was identified in 56% of VAIN and 50% of vaginal SCCs, 71.4% of VIN and 50% of vulvar SCCs. E6/E7 mRNA expression was found in one-third of VAIN and in all vaginal SCCs, 42.9% of VIN and 83.3% of vulvar SCCs. Our data indicated that HPV 16 was the commonest genotype identified in VAIN and VIN and the only genotype found in SCCs of the vagina and vulva. These findings may suggest, in accordance with other studies, that mRNA assay might be useful in triaging lesions with increased risk of progression to cancer.

Highlights

Human papillomavirus (HPV) infection of the female genital tract, is frequent worldwide and its majority is transient, while at the same time, the persistent infections caused by the oncogenic types of HPV are responsible for cancer development
HPV infection was detected in 56% of VAIN (10/18) and in 50% of vaginal squamous cell carcinomas (SCCs) (2/4)
Detectable HPV DNA from at least one of the 24 genotypes was found in 75% (6/8) of cases of vaginal lesions (VAIN I) and 40% (4/10) of VAIN II/III

Summary

Introduction

Human papillomavirus (HPV) infection of the female genital tract, is frequent worldwide and its majority is transient, while at the same time, the persistent infections caused by the oncogenic types of HPV are responsible for cancer development. This oncogenic action of HPV is a result of the transformation ability of the high-risk (hr) HPV types’ oncoproteins E6 and E7. The oncogenic properties of high-risk HPV reside in the E6 and E7 genes, which if inappropriately expressed in dividing cells deregulate cell division and differentiation [1]. The causal relation of HPV infection with development of cervical cancer has been firmly established. The above data may vary from population to population, and to our knowledge, no such analysis has been conducted in Greece

Objectives

Methods

Results

Discussion

Conclusion