Abstract
BackgroundInvasive cervical cancer is the second most common malignant tumor affecting Brazilian women. Knowledge on Human Papillomavirus (HPV) genotypes in invasive cervical cancer cases is crucial to guide the introduction and further evaluate the impact of new preventive strategies based on HPV. We aimed to provide updated comprehensive data about the HPV types’ distribution in patients with invasive cervical cancer.MethodsFresh tumor tissue samples of histologically confirmed invasive cervical cancer were collected from 175 women attending two cancer reference hospitals from São Paulo State: ICESP and Hospital de Câncer de Barretos. HPV detection and genotyping were performed by the Linear Array HPV Genotyping Test (Roche Molecular Diagnostics, Pleasanton,USA).Results170 out of 172 valid samples (99%) were HPV DNA positive. The most frequent types were HPV16 (77.6%), HPV18 (12.3%), HPV31 (8.8%), HPV33 (7.1%) and HPV35 (5.9%). Most infections (75%) were caused by individual HPV types. Women with adenocarcinoma were not younger than those with squamous cell carcinoma, as well, as women infected with HPV33 were older than those infected by other HPV types. Some differences between results obtained in the two hospitals were observed: higher overall prevalence of HPV16, absence of single infection by HPV31 and HPV45 was verified in HC-Barretos in comparison to ICESP patients.ConclusionsTo our knowledge, this is one of the largest studies made with fresh tumor tissues of invasive cervical cancer cases in Brazil. This study depicted a distinct HPV genotype distribution between two centers that may reflect the local epidemiology of HPV transmission among these populations. Due to the impact of these findings on cervical cancer preventive strategies, extension of this investigation to routine screening populations is warranted.
Highlights
Invasive cervical cancer is the second most common malignant tumor affecting Brazilian women
The present study provides an update on Human Papillomavirus (HPV) genotypes distribution in samples of Invasive cervical cancer (ICC) from patients attending two major reference cancer hospitals in São Paulo State, attempting to identify multiple infections and investigate the association between specific histological types and HPV genotypes
144 (83.7%) tumors were classified as squamous cell carcinoma (SCC) and 28 (16.3%) as adenocarcinoma (ADC)
Summary
Invasive cervical cancer is the second most common malignant tumor affecting Brazilian women. Knowledge on Human Papillomavirus (HPV) genotypes in invasive cervical cancer cases is crucial to guide the introduction and further evaluate the impact of new preventive strategies based on HPV. In Brazil, ICC is the second most common cancer among women and the National Cancer Institute (INCA), agency of the Ministry of Health responsible for cancer prevention and control policies, estimated that 17,540 new cases. HPV16 and 18 together are responsible for 70% of all ICC cases de Oliveira et al BMC Cancer 2013, 13:357 http://www.biomedcentral.com/1471-2407/13/357 globally and are the two most frequent HPV types in all geographical regions, while the third most common HPV type varies according to country and population group [10,11,12]. All them reported HPV16 and 18 as the most frequent HPV types [13,14,15,16], followed by HPV 31 and 33 [13,14], with exception of the Northeast region, where the third most common HPV type is HPV58, followed by HPV45 [16]
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