Human papillomavirus detection in Moroccan patients with bladder cancer

Noâma Berrada,Rabii Ameziane-El-Hassani,Mohammed El-Mzibri,Laïla Benbacer,Mohammed Abbar,Mohammed Attaleb,Abderrahmane Al-Bouzidi,Meriem Khyatti,Ahmed Ameur,Zineb Qmichou,Saaïd Amzazi

doi:10.3855/jidc.3068

Abstract

Human papillomavirus (HPV) is associated with more human cancers than any other virus. Many studies have investigated the association between bladder cancer and HPV but the results remain controversial. The aim of the present study is to evaluate whether HPV have an etiological role in bladder carcinogenesis among Moroccan patients. Forty-eight fresh biopsies (43 bladder tumors and 5 non-tumor samples) were collected for this purpose. Nested PCR with the consensus MY09/MY11 and GP5+/GP6+ primers was performed to detect the presence of HPV L1 gene DNA. The results showed that 52.4% of bladder cancer patients were positive for HPV. Subsequent DNA sequencing of positive cases of HPV revealed the presence of HPV16 in 95.5% of bladder tumor samples. The occurrence of HPV infection varies according to clinicopathological features, but there is no significant correlation between the viral infection and tumor stage or grade. In addition, statistical analysis demonstrated that there is no association between age or sex and HPV infection. Our data indicate for the first time that bladder tumors from Moroccan patients harbor HR-HPV genotypes, especially HPV16, and thereby suggest that this virus may play a causative role in bladder cancer.

Highlights

Human papillomavirus (HPV) is associated with more human cancers than any other virus
The pathological analysis, which was performed according to the World Health Organization (WHO) and TNM classification of malignant tumors, revealed that among the 48 cases, 42 cases were urothelial carcinomas (UC), 5 were bladder inflammatory cases, and one case was adenocarcinoma
We demonstrated the presence of HPV DNA in 52.4% of the Urothelial carcinoma (UC) cases (22/42) and in 100% of the inflammatory cases (5/5), while the one adenocarcinoma case was lacking in viral DNA

Summary

Introduction

Human papillomavirus (HPV) is associated with more human cancers than any other virus. More than 200 different HPV genotypes have been described, but Berrada et al – HPV in bladder cancer only 40 genotypes can infect the genital tract [10] and are associated with epithelial neoplasms ranging from benign common warts to malignant carcinoma of the uterine cervix [11]. According to their ability to transform epithelial cells, HPV genotypes are divided into low-risk and high-risk types. The oncogenicity of the HPV highrisk type is due to the activity of two oncoproteins, E6 and E7 [13]

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