Abstract
This study aimed to examine hTERC gene in different grades of cervical intraepithelial neoplasia (CIN) and cervical cancer, and the association between hTERC and high risk-human papillomavirus (HR-HPV) infection. Patients who underwent cervical cancer screening at the Second Affiliated Hospital of Kunming Medical University between October 2010 and December 2011 were enrolled. All patients underwent liquid-based cytology test and hybrid capture 2 (HC2) for HPV detection. hTERC was examined using fluorescence in situ hybridization (FISH). Cervical colposcopy biopsy was performed if any of the three results was positive. HC2, FISH, and pathology were compared. A total of 1200 women underwent screening, 150 patients underwent cervical biopsy: 32 in the normal group, 38 in the CIN1 group, 66 in the CIN2/3 group, and 14 in the invasive cervical cancer group. More patients had HR-HPV infection in the CIN2/3 group and ICC group compared with the CIN1 group. hTERC increased with increasing histological dysplasia. There was significant difference in hTERC positive rate between each of the three groups. More patients with hTERC gene amplification were observed in the positive HR-HPV group than in the HR-HPV negative group. In conclusion, hTERC is a potential marker for precancerous cervical cancer lesions. hTERC might be correlated with HR-HPV infection in cervical diseases.
Highlights
This study aimed to examine human telomerase RNA component (hTERC) gene in different grades of cervical intraepithelial neoplasia (CIN) and cervical cancer, and the association between hTERC and high risk-human papillomavirus (HRHPV) infection
During the study period (October 2010 to December 2011), 1200 women participated in the cervical cancer screening program and 150 (12.5%) underwent cervical biopsy, including 32 (21.3%) in the normal group, 38 (25.3%) in the CIN1 group, 66 (44.0%) in the CIN2/3 group, and 14 (9.3%) in the ICC group
The results showed that hTERC increased with increasing histological dysplasia and there was a significant difference in the hTERC positive rate between each of the three groups. 90.9% patients had HR-Human papillomavirus (HPV) infection in the CIN2/3 group, 92.9% in the ICC group and 65.8% in the CIN1 group
Summary
This study aimed to examine hTERC gene in different grades of cervical intraepithelial neoplasia (CIN) and cervical cancer, and the association between hTERC and high risk-human papillomavirus (HRHPV) infection. Long-term or repeated infection of HR-HPV may lead to unlimited proliferation of cells, greatly increasing the incidence of cervical cancer[11]. The HPV proto-oncoprotein encoded by its oncogene E6/E7 can up-regulate telomerase activity in cervical cancer and cervical intraepithelial neoplasia after HR-HPV infection, so that undifferentiated epithelial cells continue to proliferate[18]. HR-HPV infection is the initiation factor leading to reactivation of hTERC, and the combined action of the two result in cervical cells gaining unlimited proliferation ability[20]. There is a close correlation between HR-HPV infection and activation of the hTERC gene in the development of cervical cancer[21,22,23]. Increased HR-HPV and hTERC are related to more aggressive disease and may be important parts of future screening tests[21,22]
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