Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance

Abstract

Corrigendum17 February 2005free access Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance Supriya G Prasanth Supriya G Prasanth Search for more papers by this author Kannanganattu V Prasanth Kannanganattu V Prasanth Search for more papers by this author Khalid Siddiqui Khalid Siddiqui Search for more papers by this author David L Spector David L Spector Search for more papers by this author Bruce Stillman Bruce Stillman Search for more papers by this author Supriya G Prasanth Supriya G Prasanth Search for more papers by this author Kannanganattu V Prasanth Kannanganattu V Prasanth Search for more papers by this author Khalid Siddiqui Khalid Siddiqui Search for more papers by this author David L Spector David L Spector Search for more papers by this author Bruce Stillman Bruce Stillman Search for more papers by this author Author Information Supriya G Prasanth, Kannanganattu V Prasanth, Khalid Siddiqui, David L Spector and Bruce Stillman The EMBO Journal (2005)24:1092-1093https://doi.org/10.1038/sj.emboj.7600624 This article corrects the following: Cullin-based ubiquitin ligases: Cul3–BTB complexes join the family08 April 2004 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Correction to: The EMBO Journal (2004) 23, 1681–1687. doi:10.1038/sj.emboj.7600186 Due to an author error, the X-axis in Figure 3D was mislabelled as INA instead of DNA. The correct figure is reproduced below. Figure 1.Orc2 depletion has dual execution points. (A) Immunoblot of whole-cell extract from cells transfected with Orc2 siRNA duplex or control luciferase siRNA and harvested at different time points. Cells were transfected at 0, 24 and 48 h at about 20% starting confluence. Efficacy of RNAi was assessed by immunoblotting to detect Orc2. a-Tubulin levels were used as loading controls. Orc3 levels were also modulated and, with the reappearance of Orc2 in the cells at 144 h, the levels of Orc3 also increased. (B) Orc2 depletion at 72 h post siRNA shows morphologically two distinct populations of cells: the flat adherent cells and the rounded mitotic cells. (C) DNA content of control and Orc2 siRNA-treated cells at 48 h was determined by flow cytometry. Note a broader S phase and an increase in G2/M in Orc2 siRNA-treated cells. (D) Two-dimensional FACS to assess BrdU incorporation (10 min pulse) and DNA content by propidium iodide (PI) was assessed by flow cytometry in control and Orc2-depleted cells at 72 h. Note an increase in G2/M and accumulation of cells in S phase without incorporation of BrdU (see arrow) in Orc2-depleted cells. (E) Histogram showing the percentage of cells incorporating BrdU during a 24 h label at different time points after transfection, with Orc2 (gray bars) or luciferase control (white bars) siRNA duplexes. BrdU incorporation was allowed for 24 h to ensure that all cells had a chance to pass through at least one S phase. Download figure Download PowerPoint The authors apologize for this error. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 24,Issue 5,March 9, 2005Crinoids - also called Feather Stars, appear as a dense mass of arms hidden in the smallest crevices, holes or other hiding places of tropical reefs during daytime. The picture was captured at Ellaidhoo, a small Maldive island of the Ari atoll (Indo-Pacific Ocean) with a Nikonos RS/50mm macro lens on Kodak Elitechrome 100 ASA colour slide film. The photographer, Wolfgang Seifarth, is a molecular biologist at the University of Heidelberg, Germany, where he studies the importance of endogenous retroviral elements (HERV) in the human genome. Wolfgang Seifarth also has a strong interest in marine biology field research and taxonomy, and underwater photography. His image of the crinoids was selected as the winner of the EMBO Journal Cover Contest 2005. Volume 24Issue 59 March 2005In this issue FiguresRelatedDetailsLoading ...