Abstract
The extensive need for hard tissue substituent greatly motivates development of suitable allogeneic grafts for therapeutic recreation. Different calcium phosphate phases have been accepted as scaffold’s components with positive influence on osteoinduction and differentiation of human mesenchymal stem cells, in terms of their higher fraction within the graft. Nevertheless, the creation of unlimited nutrients diffusion through newly formed grafts is of great importance. The media flow accomplished by perfusion forces can provide physicochemical, and also, biomechanical stimuli for three-dimensional bone-construct growth. In the present study, the influence of a different scaffold’s composition on the human mesenchymal stem cells (hMSCs) differentiation performed in a U-CUP bioreactor under perfusion conditioning was investigated. The histological and immunohistochemical analysis of cultured bony tissues, and the evaluation of osteogenic genes’ expression indicate that the lower fraction of in situ formed hydroxyapatite in the range of 10–30% within chitosan scaffold could be preferable for bone-construct development.
Highlights
The development of functional allogeneic tissue constructs requires the coordination of cell adhesion, growth, differentiation, and organization into specific tissue architecture
The synthesis of chitosan-hydroxyapatite (Cht-HA) composites with different amount of hydroxyapatite precursors, as well as porous structures, was done according to our previous work [23]: to obtain required HA percentage, appropriate amounts of calcite were suspended in chitosan solution
The chitosan-hydroxyapatite scaffolds represent a favorable microenvironment for tissue penetration and ingrowth, as indicated by histological analysis of cultured grafts
Summary
The development of functional allogeneic tissue constructs requires the coordination of cell adhesion, growth, differentiation, and organization into specific tissue architecture. The engineering of a suitable stem cell microenvironment becomes an important approach in regenerative medicine [1]. Mesenchymal stem cells (MSCs) isolated from adult bone marrow are recognized as self-renewing cells capable of differentiating into multiple different cell phenotypes, providing many advantages in tissue regeneration strategies [2,3]. Biomaterial scaffolds and dynamic bioreactors, such as perfusion bioreactor, represent two important components in the formation of suitable microenvironments providing controlled patterns of biochemical and biomechanical signaling [5]. Finding the proper synergy between scaffolds features and perfusion conditioning can direct MSCs behavior during the graft development
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
