Abstract
The aim of this work was to investigate the anticancer cytotoxic effects of natural compound, subamolide E, on the human skin cancer melanoma A375.S2 cells. Subamolide E was isolated from Cinnamomum subavenium and demonstrated cytotoxicities in cell growth assay at concentration rages from 10–100µM at 24h. Propidium iodide staining and flow cytometry analyses were used to evaluate cell cycle distribution and found that subamolide E caused DNA damage in sub G1 phase with a dose-dependent manner after 24h of treatment. According to the Western blot result, subamolide E-treated cells were with the increasing of caspase-dependent apoptotic proteins to induce related pathway mechanisms. Subamolide E also showed that anti-migratory activities of A375.S2 cells on wound healing assay. Finally, subamolide E demonstrated minor cytotoxicities to human normal skin cells (keratinocytes, melanocytes and fibroblasts), therefore, it is a potential chemotherapeutic agent against skin cancer.
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