Abstract
Tear stability decreases with increasing age and the same signs of instability are exacerbated with dry eye. Meibum lipid compositional changes with age provide insights into the biomolecules responsible for tear film instability. Meibum was collected from 69 normal donors ranging in age from 0.6 to 68 years of age. Infrared spectroscopy was used to measure meibum lipid phase transition parameters. Nuclear magnetic resonance spectroscopy was used to measure lipid saturation. Increasing human meibum lipid hydrocarbon chain unsaturation with age was related to a decrease in hydrocarbon chain order, cooperativity, and in the phase transition temperature. The change in these parameters was most dramatic between 1 and 20 years of age. Meibum was catalytically saturated to determine the effect of saturation on meibum lipid phase transition parameters. Hydrocarbon chain saturation was directly related to lipid order, phase transition temperature, cooperativity, changes in enthalpy and entropy, and could account for the changes in the lipid phase transition parameters observed with age. Unsaturation could contribute to decreased tear film stability with age.
Highlights
Tear lipids, mostly from the Meibomian gland and a minor amount from sebaceous glands [1], may be important for tear stability [2,3,4]
Tear break-up time is even lower (5 s) in adults with Meibomian gland dysfunction [16,17,18,19,20,21,22]. This project is an extension of previous nuclear magnetic resonance (NMR) [23,24] and Fourier transform infrared (FTIR) [1,24,25,26,27,28] spectral studies relating age with meibum composition, structure, This project is an extension of previous nuclear magnetic resonance (NMR) [23,24] and Fourier
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Summary
Mostly from the Meibomian gland and a minor amount from sebaceous glands [1], may be important for tear stability [2,3,4]. Tear break-up time is even lower (5 s) in adults with Meibomian gland dysfunction [16,17,18,19,20,21,22]. This project is an extension of previous nuclear magnetic resonance (NMR) [23,24] and Fourier transform infrared (FTIR) [1,24,25,26,27,28] spectral studies relating age with meibum composition, structure, This project is an extension of previous nuclear magnetic resonance (NMR) [23,24] and Fourier. Texhaismwinaestahneirmelpartoiovnesmhiepnst boevtewr etehne hpyrdevroiocuasrbsotnudcyhawinheorredeornalyndnathtievelemveelibouf msatwuraasticoonm. pTahriesdwwasithanmimeipburomvetmhaetnwt oavse1r0t0h%e psaretuviroautesdst[u25d]y, awlheevreel othnalyt insantiovtepmhyesibioulmogwicals. cTohmispsatrueddywpirthovmideeibsuinmsitghhattswinatso1h0o0w% tshaetuirnactreedas[e25in], amleivbeulmthalitpiisdnsoatuprhaytisoionlcoagnicbael. rTehlaistesdtutodythperobvsiderevseidnsdigehcrtesaisnetoinhtoewartfhileminsctraebaislietyinwmitheibaguem. lipid saturation can be related to the observed decrease in tear film stability with age
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