Human Mammary Cell Resource.

Abstract

Abstract : My laboratory has generated a human mammary epithelial cell (HMEC) system for studies on normal and aberrant growth control mechanisms. To facilitate widespread use of HMEC, we have provided cells and cell culture expertise to over 150 laboratories worldwide. To optimize use of this resource, we developed a complex database for storage and retrieval of information on the HMEC, and newsletters for communication among those using llMEC. The purpose of this grant was to (1) upgrade and expand the features of this database, (2) ensure that all computer and written records were readily understandable to others besides the PI, (3) create written histories of complicated cell lineages and enter them into the database, (4) develop a web site to disseminate information about HMEC culture and to foster communication among laboratories using HMEC. Most work on the database upgrade was completed in year 1. For years 2 and 3, old records were entered into the database and work was begun compiling cell histories and making written records more accessible. A web site on HMEC was launched in year 2 and has been upgraded in year 4. This work is largely proceeding as planned, although the history compilation will require more time than this granting period.