Abstract
LYMPHOCYTES from peripheral blood can be stimulated to undergo blast-like transformation when cultured in vitro1. This reaction is revealed by many techniques including direct morphological observations, the appearance of mitotic figures in dividing cells, and specific staining or autoradiography for the various biochemical processes involved in cell division2. Although any of these techniques is satisfactory, the percentage of transforming cells in many specific immune reactions is low3, making standardization both tedious and difficult. In addition, in culture, a small percentage of lymphocytes undergo blast transformation in unstimulated controls either spontaneously or possibly because of prior sensitization of the individual to one of the products in the tissue-culture medium4.
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