Abstract
Here, we have described the dataset relevant to the A549 cellular proteome changes after exposure to either titanium dioxide or carbon black particles as compared to the non-exposed controls, “Proteomic changes in human lung epithelial cells (A549) in response to carbon black and titanium dioxide exposures” (Vuong et al., 2016) [1]. Detailed methodologies on the separation of cellular proteins by 2D-GE and the subsequent mass spectrometry analyses using MALDI-TOF-TOF-MS are documented. Particle exposure-specific protein expression changes were measured via 2D-GE spot volume analysis. Protein identification was done by querying mass spectrometry data against SwissProt and RefSeq protein databases using Mascot search engine. Two-way ANOVA analysis data provided information on statistically significant A549 protein expression changes associated with particle exposures.
Highlights
We have described the dataset relevant to the A549 cellular proteome changes after exposure to either titanium dioxide or carbon black particles as compared to the non-exposed controls, “Proteomic changes in human lung epithelial cells (A549) in response to carbon black and titanium dioxide exposures” (Vuong et al, 2016) [1]
Particle exposure-specific protein expression changes were measured via 2.5. Two-dimensional gel electrophoresis (2D-GE) spot volume analysis
Protein identification was done by querying mass spectrometry data against SwissProt and RefSeq protein databases using Mascot search engine
Summary
MALDI-TOF-TOFMS analysis of tryptic-digested peptides from 2D-GE gel spots for protein identification was done using a Bruker Autoflex III Smartbeam instrument. Particle-induced A549 protein expression changes along with protein identification help to characterize toxicity mechanisms and related altered cellular functions. These data and the toxicoproteomic approach are promising in the study of toxicity of environmental particles and engineered nano-materials. The first is the 2D-GE protein expression dataset associated with A549 cells exposed to TiO2 and CB particles (Supplentary Table 1). The second dataset describes the corresponding protein identification results based on MALDI-TOF-TOF-MS analyses (Supplentary Table 2). The third dataset reveals the 2D-GE protein spots that significantly changed due to particle exposures (Supplentary Table 3)
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