Human liver cDNA clones encoding proteolipid subunit 9 of the mitochondrial ATPase complex

Abstract

Clones encoding the proteolipid subunit 9 of the mitochondrial ATPase complex have been isolated from a λgt10 library of human liver cDNA sequences, using a probe of Neurospora crassa cDNA encoding subunit 9. From nucleotide sequence analysis it is concluded that the amino acid sequence of mature human subunit 9 is identical to that of its bovine counterpart. By comparing the sequence of two cDNA clones (denoted human 1 and 2) to those of two bovine cDNA clones (denoted P1 and P2) recently described by Gay and Walker (EMBO J. 4, 3519–3524, 1985) it is evident that there are close sequence relationships between human 1 and bovine P1, and between human 2 and bovine P2, although both human clones are truncated at their 5′-ends. Thus, as in bovine cells there appears to be at least two human genes encoding subunit 9.