Human Inter-alpha-Trypsin-Inhibitor: Characterization and partial nucleotide sequencing of a light chain-encoding cDNA

Abstract

A synthetic Inter-α-Trypsin-Inhibitor (ITI)-specific oligonucleotide probe was used to isolate a clone from a human liver cDNA library. The amino-acid sequence deduced from partial nucleotide sequencing of the corresponding cDNA insert perfectly matched a known ITI sequence, apart from an as yet unreported C-terminal dipeptide. Hybridization on Northern blots evidenced that this cDNA insert originated from an ITI light chain-encoding mRNA whose size was estimated to be 1 300 bases.