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Abstract
Human gyrovirus (HGyV) is a recent addition to the list of agents found in humans. Prevalence, biologic properties, and clinical associations of this novel virus are still incompletely understood. We used qualitative PCRs to detect HGyV in blood samples of 301 persons from Italy. HGyV genome was detected in 3 of 100 solid organ transplant recipients and in 1 HIV-infected person. The virus was not detected in plasma samples from healthy persons. Furthermore, during observation, persons for whom longitudinal plasma samples were obtained had transient and scattered presence of circulating HGyV. Sequencing of a 138-bp fragment showed nucleotide identity among all the HGyV isolates. These results show that HGyV can be present in the blood of infected persons. Additional studies are needed to investigate possible clinical implications.
Highlights
Human gyrovirus (HGyV) is a recent addition to the list of agents found in humans
In 2011, Sauvage et al reported the discovery of a novel virus in human skin specimens and named it human gyrovirus (HGyV) [1]
Like related animal viruses, chicken anemia virus (CAV) infects a large range of cell types and causes a variety of pathologies and increased death in young chicken [11]
Summary
Author affiliations: Pisa University Hospital, Pisa, Italy A sample was considered rtPCR positive when HGyV DNA was detected in 2 of 3 replicas and when amplifications were specific as determined by 2% agarose gel electrophoresis. A 178-bp fragment was amplified by a nested PCR format, described by Sauvage et al [1] with modifications This PCR was performed for 25 cycles with sense primer HGyV-OF (5′-CAAAATCGGAGGCCCTAACCC-3′) and antisense primer HGyV-OR (5′-ATGCCTGAATAGCTGC CAGCC-3′) under the following conditions: denaturation at 94°C for 60 s, annealing at 55°C for 60 s, and extension at 72°C for 45 s. The product of this reaction (10 μL). Nucleotide sequences were aligned with the only sequence available at GenBank at the time of writing and by using the ClustalW algorithm included in BioEdit version 7.0.9.0 (www.clustal.org)
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