Abstract
We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection. Patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to A. phagocytophilum antigens propagated in THP-1 rather than HL60 cells. Unless both cell lines are used for serodiagnosis of rickettsiosis-like infections, cases of human granulocytic anaplasmosis could go undetected.
Highlights
We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection
In immunofluorescence assay (IFA), IgM and/ or IgG from serum samples from the case-patients reacted with A. phagocytophilum cultured in THP-1 rather than HL60 cells, and seroconversion was stronger in convalescent-phase serum samples (Table 2)
Western blot analysis further confirmed the specific reaction to the 44-kDa outer membrane proteins (P44s) of A. phagocytophilum cultured in THP-1 cells and/or to the recombinant P44-1 in serum samples (Figure 2, Table 2)
Summary
We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection. Patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to A. phagocytophilum antigens propagated in THP-1 rather than HL60 cells. Multiplex nested first-step PCR for SFG rickettsiae and O. tsutsugamushi was performed by using the following primers: RO-1F Multiplex nested second-step PCR for O. tsutsugamushi 16S rDNA was performed by using the following primers: O-2F (5'-GACATGGTAGTCGCGAAAAATG-3')
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