Human Gingival Tissue Reactions to Silk and Expanded Polytetrafluoroethylene Sutures

Abstract

Wound closure by suturing is a critical and significant event in general as well as in oral surgery. Information regarding tissue reactions to different suture materials appears incomplete and inconsistent, particularly in humans. Thus, the purpose of the present study was to evaluate clinically and histologically tissue reactions to silk and expanded polytetrafluoroethylene (ePTFE) suture materials placed in human oral tissues. Twelve patients undergoing periodontal treatment, who had bilateral periodontal defects in the maxillary premolar and molar region scheduled for resective surgery, were included. In each patient, one single interrupted ePTFE suture (CV-5) and one single interrupted silk suture (4-0) were inserted in gingival tissues in contralateral jaw quadrants 10 days prior to scheduled surgery and a second set of sutures was inserted at 7 days prior to surgery. After placement, the length of the suture embedded in the tissue as well as the slack of the suture loop were measured and tabulated. The measurements were repeated on the day of surgery. At this time biopsy specimens, including the suture loop and surrounding tissue, were harvested and processed for histologic analysis. The inflammatory response was evaluated by measuring the thickness of the perisutural epithelium and the diameter of the connective tissue infiltrate, by estimating the proportion of inflammatory cells to epithelial cells, and by recording the presence or absence of bacterial plaque along the suture track. At 7 days, silk sutures showed a significantly higher degree of slack of the suture loop than did ePTFE sutures (P= 0.02), while the difference was not significant at 10 days (P= 0.21). The mean change of "tissue bite" was greater for silk compared to ePTFE at 7 as well as at 10 days. However, none of the differences were statistically significant (P= 0.43). The thickness of the perisutural epithelium increased significantly from 7 to 10 days for both suture materials, while the difference in proportion of inflammatory cells to epithelial cells was not significant (P= 0.43). An overall significantly greater diameter of connective tissue infiltrate was present around the silk sutures. Bacterial plaque was detected in 10 of 11 silk and four of 11 ePTFE suture channels at 7 days, and eight of 10 and four of 11 suture channels at 10 days. The results revealed that placement of sutures in gingival tissues elicits an inflammatory reaction and that the magnitude of this reaction may vary with the suture material used. Braided silk sutures apparently cause a more extensive inflammatory tissue reaction in an environment characterized by moisture and infectious potential.