Abstract
BackgroundThe possibility of using stem cells for regenerative medicine has opened a new field of investigation. The search for sources to obtain multipotent stem cells from discarded tissues or through non-invasive procedures is of great interest. It has been shown that mesenchymal stem cells (MSCs) obtained from umbilical cords, dental pulp and adipose tissue, which are all biological discards, are able to differentiate into muscle, fat, bone and cartilage cell lineages. The aim of this study was to isolate, expand, characterize and assess the differentiation potential of MSCs from human fallopian tubes (hFTs).MethodsLineages of hFTs were expanded, had their karyotype analyzed, were characterized by flow cytometry and underwent in vitro adipogenic, chondrogenic, osteogenic, and myogenic differentiation.ResultsHere we show for the first time that hFTs, which are discarded after some gynecological procedures, are a rich additional source of MSCs, which we designated as human tube MSCs (htMSCs).ConclusionHuman tube MSCs can be easily isolated, expanded in vitro, present a mesenchymal profile and are able to differentiate into muscle, fat, cartilage and bone in vitro.
Highlights
The possibility of using stem cells for regenerative medicine has opened a new field of investigation
We and others have recently shown that the umbilical cord, dental pulp, orbicular oris muscle and adipose tissue are a very rich source of mesenchymal stem cells (MSCs) able to differentiate into muscle, cartilage, bone and adipose cell lineages [7,10,1215]
The majority of cells expressed high levels of adhesion markers (CD29, CD44 and CD90) and MSCs markers
Summary
The possibility of using stem cells for regenerative medicine has opened a new field of investigation. It has been shown that mesenchymal stem cells (MSCs) obtained from umbilical cords, dental pulp and adipose tissue, which are all biological discards, are able to differentiate into muscle, fat, bone and cartilage cell lineages. Adult mesenchymal stem cells (MSCs) are typically defined as undifferentiated multipotent cells endowed with the capacity for self-renewal and the potential to differentiate into several distinct cell lineages [1]. These progenitor cells which constitute a reservoir found within the connective tissue of most organs are involved in the maintenance and repair of tissues throughout the postnatal life of an individual. Endometrial and menstrual blood-derived stem cells were recently isolated and showed the ability to differentiate into cell types of the three germ layers [17,18,19,20,21,22,23]
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