Abstract
Human chymase is known to function as a chemoattractant for human leukocytes. To investigate the mechanism of the chymase-induced cell migration, change in intracellular calcium concentration ([Ca 2+]i) was examined in human polymorphonuclear (PMN) cells using Fluo-3 as a fluorescent Ca 2+ indicator. Treatment of PMN cells with human chymase caused [Ca 2+]i elevation in a concentration-dependent manner. Depletion of extracellular Ca 2+ from the medium partially attenuated the chymase-induced [Ca 2+]i increase, showing that both Ca 2+ influx and Ca 2+ release from internal stores might be involved in the [Ca 2+]i response. Pretreatment of the cells with pertussis toxin completely blocked the chymase-induced [Ca 2+]i signal, suggesting an involvement of G protein in the chymase-mediated [Ca 2+]i elevation. The data in the present study raise the possibility that the chymase-induced cell migration is mediated by the [Ca 2+]i elevation, which might be caused by stimulation of a G-protein-coupled receptor such as protease-activated receptors (PARs).
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