Abstract
To detect hCG in spent embryo culture media at day 2 after intracytoplasmic sperm injection and to assess the relationship of hCG to embryo development. Experimental study. Fertility center and clinical diagnostic laboratory. A total of 102 spent culture media from day 2 human embryos and corresponding unexposed media for blank control. The culture media samples were tested for hCG by ELISA. Quantity of hCG produced by embryos and correlation with the embryos' developmental status. hCG was found in 93 of 102 culture media tested by enzyme-linked immunosorbent assay. The correlation analysis revealed that the concentration of hCG was independent of embryo developmental status. The ability to detect hCG from day 2 spent culture media may be used as a marker for embryo competence.
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