Abstract
Eight serially transplantable human breast-cancer xenograft lines have been established in immune-suppressed mice. Specimens from 102 primary and secondary lesions obtained at surgery from 80 patients were implanted into mice immune-suppressed by thymectomy and whole-body irradiation. A number of variations of implantation site, transplantation technique, method of immune suppression and hormonal manipulation of the host were tried in an attempt to increase the take rate, but without success. The 8 lines established have been serially transplanted into further immune-suppressed mice for at least 2 passages, and appear to maintain characteristic human histopathology, chromosome number and tumour-marker production. None of the tumours show hormone sensitivity. The poor take rate may be a reflection of the biological nature of breast cancer rather than a failure of the immune-deprivation technique, as many other human tumours grow well as xenografts in this system.
Highlights
Summary.-Eight serially transplantable human breast-cancer xenograft lines have been established in immune-suppressed mice
TION of human tumours to immunesuppressed rodents was first described nearly 30 years ago (Toolan, 1951) a variety of tumours of different histological types have been established as transplantable xenograft lines in both artificially immune-suppressed and congenitally athymic mice
If serial transplantation is considered a necessary characteristic of a successful xenograft, published take rates vary from zero (Gershwin et al, 1977) to 1355% (Giovanella et al, 1976) using nude mice
Summary
Summary.-Eight serially transplantable human breast-cancer xenograft lines have been established in immune-suppressed mice. TION of human tumours to immunesuppressed rodents was first described nearly 30 years ago (Toolan, 1951) a variety of tumours of different histological types have been established as transplantable xenograft lines in both artificially immune-suppressed and congenitally athymic (nude) mice. The use of this model system in experimental chemotherapy and in the study of various aspects of human tumour biology has been described (Cobb & Mitchley 1974; Pickard et al, 1975;, Povlsen & Jacobsen, 1975; Steel, 1978). The xenograft lines reported are the first serially transplantable human breast xenografts to be established and maintained in immune-suppressed as opposed to nude mice
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