Abstract
When it comes to regenerative medicine, mesenchymal stem cells (MSCs) are considered one of the most promising cell types for use in many cell therapies and bioengineering protocols. The International Society of Cellular Therapy recommended minimal criteria for defining multipotential MSC is based on adhesion and multipotency in vitro, and the presence or absence of select surface markers. Though these criteria help minimize discrepancies and allow some comparisons of data generated in different laboratories, the conditions in which cells are isolated and expanded are often not considered. Herein, we propose and recommend a few procedures to be followed to facilitate the establishment of quality control standards when working with mesenchymal progenitors isolation and expansion. Following these procedures, the classic Colony-Forming Unit-Fibroblast (CFU-f) assay is revisited and three major topics are considered to define conditions and to assist on protocol optimization and data interpretation. We envision that the creation of a guideline will help in the identification and isolation of long-term stem cells and short-term progenitors to better explore their regenerative potential for multiple therapeutic purposes.
Highlights
To minimize discrepancies and inconsistencies, and allow comparison of data generated in different laboratories, members of the International Society of Cellular Therapy (ISCT) (Horwitz et al, 2005) have recommended minimal criteria for defining multipotential mesenchymal stem cells (MSCs)
Growing evidence indicates an intimate relationship between MSCs and those cells identified as pericytes, since these two populations demonstrate similar behavior and potential in vitro and in vivo (Shi and Gronthos, 2003; Sacchetti et al, 2007; Taichman et al, 2010; Péault, 2012)
Mesenchymal-like stem cells and progenitors have been isolated from several tissues, but adipose tissue and bone marrow are usually indicated as most promising sources of these cells by those working in the cell therapy and bioengineering fields (Da Silva Meirelles et al, 2006; Crisan et al, 2008b; Corselli et al, 2011)
Summary
To minimize discrepancies and inconsistencies, and allow comparison of data generated in different laboratories, members of the International Society of Cellular Therapy (ISCT) (Horwitz et al, 2005) have recommended minimal criteria for defining multipotential mesenchymal stem cells (MSCs). They showed that when bone marrow cells were placed into culture at low density, a few of them adhered, proliferated, and gave rise to colonies of fibroblast-like cells (CFU-f).
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