Abstract
Lunasin is a bioactive peptide with anticancer properties originally isolated from soy. The objective of this study was to assess the presence of lunasin in blood of men fed soy protein products. Five healthy male subjects age 18‐25 were fed with 50 g of soy protein for 5 days, blood was taken at the beginning of the study and at 30 min and 1 h after soy protein ingestion. Lunasin was isolated from plasma by ion exchange chromatography using magnetic beads and quantified by ELISA. At day 5, lunasin in plasma ranged from 50.2 to 110.6 ng/ml (average, 66.0 ± 25.4 ng/ml) after 30 min and 33.5 to 122.7 ng/ml (average, 71.0 ± 32.8 ng/ml) after 1 h. An average 4.5% lunasin absorption (range 2.2‐7.8%) was estimated from the total lunasin ingested. MALDI‐TOF profiling showed a 5 kDa peptide similar to synthetic lunasin in plasma samples of subjects fed soy protein while it was absent in the baseline plasma samples from the same individuals. LC/MS‐MS analysis also showed the presence of amino acids from lunasin in plasma samples after soy intake but not in plasma samples after a wash out period and before soy consumption. The results of the present study suggest that lunasin is bioavailable in humans; an important requirement for its anticancer potential.Supported by ACES Global Connect, University of Illinois and CONACYT Mexico
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