Human autologous rosette-forming cells: I. Expression of cell surface antigens in relation to age and lymphoid organ distribution

Abstract

Autologous rosette-forming cells (auto-RFC) were characterized with monoclonal antibodies to various cell surface antigens using a technique combining immunofluorescence and rosette formation. In peripheral blood, auto-RFC were T cells ( Leu 1 + OKT3 + ) the majority being derived from the helper/inducer subset ( Leu 3 a + OKT4 + ). A small proportion of the circulating auto-RFC were Leu 2 a + OKT8 + and virtually none of them bore T10, T6, and DR antigens or peanut agglutinin (PNA) receptors. In the elderly, the percentages of Leu 3a + auto-RFC increased significantly along with the augmentation of the Leu 3a + circulating pool. After Con A stimulation of peripheral blood lymphocytes the autorosette population was expanded and therefore their phenotype was again that of helper cells. In the thymus, high levels of autorosettes are found (30 to 50%). Simple or double labeling of the rosetting cells with various monoclonal antibodies permitted the confirmation of the existence of distinct thymocyte subpopulations and moreover to identify the location of the auto-RFC in the intrathymic differentiation scheme. Nearly 70% of the rosetting cells were derived from common thymocytes, those cells defined by the coexpression of T10, T6, T4, and T8 antigens whether or not they were also stained by OKT3 antibodies. The remaining auto-RFC were found with similar frequency among the T4 + and T8 + mature thymocytes. In the spleen low percentages of auto-RFC were found and the majority resided in the Leu 3 a + OKT4 + population, similarly to peripheral blood autorosettes. Taken together, these data suggest that the expression of autologous erythrocyte receptors is acquired in the thymus and is gradually lost during T-cell maturation.