Abstract
Bone volume inadequacy is an emerging clinical problem impairing the feasibility and longevity of dental implants. Human bone marrow mesenchymal stem cells (HBMSCs) have been widely used in bone remodeling and regeneration. This study examined the effect of long noncoding RNAs (lncRNAs)-H19 on the human amnion-derived mesenchymal stem cells (HAMSCs)-droved osteogenesis in HBMSCs. HAMSCs and HBMSCs were isolated from abandoned amniotic membrane samples and bone marrow. The coculture system was conducted using transwells, and H19 level was measured by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). The mechanism was further verified. We here discovered that osteogenesis of HBMSCs was induced by HAMSCs, while H19 level in HAMSCs was increased during coculturing. H19 had no significant effect on the proliferative behaviors of HBMSCs, while its overexpression of H19 in HAMSCs led to the upregulated osteogenesis of HBMSCs in vivo and in vitro; whereas its knockdown reversed these effects. Mechanistically, H19 promoted miR-675 expression and contributed to the competitively bounding of miR-675 and Adenomatous polyposis coli (APC), thus significantly activating the Wnt/β-catenin pathway. The results suggested that HAMSCs promote osteogenic differentiation of HBMSCs via H19/miR-675/APC pathway, and supply a potential target for the therapeutic treatment of bone-destructive diseases.
Highlights
Bone volume inadequacy is a crucial problem among aged patients requiring dental implants
LncRNA-H19 expression in human amnion-derived mesenchymal stem cells (HAMSCs) increases with the HAMSCs-droved osteogenesis
LncRNA-H19 expression in HAMSCs has no effects on Human bone marrow mesenchymal stem cells (HBMSCs) proliferation
Summary
Bone volume inadequacy is a crucial problem among aged patients requiring dental implants. Mesenchymal stem cells (MSCs) possess multi-potentiality and selfrenewal properties, which have been extensively applied in the treatment of different degenerative conditions, including bone defects [1]. Human bone marrow mesenchymal stem cells (HBMSCs) are the best characterized multipotent adult stem cells; HBMSCs possess self-renewal capacity, low antiinflammatory properties, and less risk of malignant www.aging-us.com transformation during in vitro amplification [2]. HBMSCs have various disadvantages, such as high traumatic response, limited availability and reduced stemness during ageing [3]. Some pathological status has direct detrimental effects on HBMSCs, which remarkably influence the cell retention and survival at the target region. Studies have suggested that human amnion-derived mesenchymal stem cells (HAMSCs) may be characterized by MSCs features, and show embryonic stem cells-like phenotypic characteristics [4]. HAMSCs could be regarded as an alternative method for bone regeneration
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