Abstract
Seawater (SW) immersion can increase the damage of skin wounds and produce refractory wounds. However, few studies have been conducted to investigate the mechanisms of SW immersion on skin wounds. In our current study, we investigated the effect of human adipose-derived stem cells (hADSCs) on the repair of SW-treated full-thickness skin wounds and the underlying mechanisms. The results showed that SW immersion could reduce the expression of EGF and suppress the activation of the MEK/ERK signaling pathway. At the same time, the proliferation and migration of skin stem cells were inhibited by SW immersion, resulting in delayed wound healing. However, hADSCs significantly accelerated the healing of SW-immersed skin wounds by promoting cell proliferation and migration through the aforementioned mechanisms. Our results indicate a role for hADSCs in the repair of seawater-immersed skin wounds and suggest a potential novel treatment strategy for seawater-immersed wound healing.
Highlights
Chronic wounds are wounds that do not reach anatomical and functional integrity within 30 days after injury [1]
To confirm that human adipose-derived stem cells (hADSCs) were successfully isolated from human adipose tissue, hADSCs were induced to differentiate into adipocytes and osteoblasts, and the adipogenic differentiation and osteogenic differentiation ability of cells was confirmed by alizarin red S staining of osteoblasts (Figure 3(b)) and oil red O staining of adipocytes (Figure 3(c)), respectively
The above characteristics were consistent with the previous research results, indicating that we obtained high-quality hADSCs
Summary
Chronic wounds are wounds that do not reach anatomical and functional integrity within 30 days after injury [1]. Obesity, persistent infection, and the use of corticosteroids can make skin wounds difficult to heal and can lead to chronic skin wounds, which may eventually lead to serious consequences such as infection, amputation, and even death [2, 3]. Seawater (SW) immersion is a common cause of chronic wounds in people living in coastal areas and involved in ocean navigation. Global SW has an average salinity of 34.7 and a pH of 8-8.4, which is a pronounced hyperosmotic alkaline state. The above characteristics mean that when skin wounds are soaked in SW for a long time, they become prone to tissue necrosis and infection, prolonging the healing time of the skin wounds and causing chronic wounds. There have been rare reports on the effects of SW on the wounds of full-thickness skin and the mechanism of its occurrence
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
