Abstract
BackgroundA keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury. To date, no effective cure has been identified. As multipotent stem cells, human adipose-derived stem cells (ADSCs) may show the possibility for curing diseases such as fibrosis. This study sought to explore the potential role of human ADSCs in curing keloids.MethodsAfter culture in conditioned medium, gene and protein expression of keloid fibroblasts was examined using real-time polymerase chain reaction (RT-PCR) and Western blotting, while analysis of the cell cycle was used to measure the proliferative properties of the cells. Furthermore, ex vivo explant cultures were used to test the effects of ADSC-conditioned medium (ADSC-CM) on CD31+ and CD34+ expression in keloid tissue.ResultsOur experimental results show that ADSC-CM was able to attenuate extracellular matrix-related gene expression as well as decrease protein expression. Cell proliferation was significantly suppressed in our study. CD31+ and CD34+ vessels in ex vivo explants were reduced by 55% and 57% in treatment groups compared with control groups.ConclusionsHuman ADSC-CM significantly inhibited keloid fibroblast-related bioactivities.
Highlights
A keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury
Characterization of Adipose-derived stem cell (ADSC) As reported previously [13], human ADSCs displayed positive staining for the specific mesenchymal stem cell surface markers CD29 (100%), CD44 (99.4%), CD105 (85.2%), and CD90 (99.8%), and negative staining for the hematopoietic stem cell surface markers CD31 (0.1%), CD34 (0.1%), and CD45 (0.1%) (Fig. 1a)
The results demonstrated that the isolated ADSCs showed typical ADSC characteristics
Summary
A keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury. Human adipose-derived stem cells (ADSCs) may show the possibility for curing diseases such as fibrosis. This study sought to explore the potential role of human ADSCs in curing keloids. A keloid caused by any traumatic stimulus is characterized by hyperproliferation of fibroblasts, excessive deposition of extracellular matrix (ECM), including collagen 1 (COL1) and collagen 3 (COL3), and constant invasion of normal tissue [1]. MSCs from adipose tissue may be easier to obtain and expand rapidly in vitro to generate an effective dose. Human adiposederived stem cells (ADSCs) have the same properties as MSCs from bone marrow [8, 9], and are currently being considered as potential therapeutic strategies for a number of diseases
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