Hubungan antara Genotyping DNA Human Pappillomavirus (HPV) dengan Respons Terapi Radiasi pada Adenokarsinoma Serviks

Abstract

The object of this study to know the type of DNA Human Papilloma Virus genotyping in adenocarcinoma cervical cancer,espescially in Cipto Mangunkusomo Hospital and Cancer Centre Dharmais Hospital. The importance of humanpapilloma virus (HPV) infection in the outcome of cervical cancer after radiotherapy remains unknown. Our studyexplored whether the HPV status of tumors and also persistence of the HPV infection is related with the outcome ofradiotherapy in patients with cervical cancer.The biopsy cervix samples taken from 12 patients with Adenocarcinoma cervical cancer (Stage IIB-IIIB) that met in theinclusion criteria. The HPV genotyping examination was conducted twice, before and 3 month after radiation therapy. Thesubjects treated by radiation therapy without sensitizer according to standard procedures. After underwent completeradiation, response of radiation therapy was conducted by clinical assessment and repeated HPV genotyping test.Result: a total of 12 patients had been collected in this study. From this sample, there were found HPV-positive tumors in91,7% (11 cases ) of patients, with the details of a single infection of 75% and 16,7% multiple infections. Based on the typeof HPV type 18 was obtained (66,7%), type 45: 8,3%. Persistent infection with HPV after radiation encountered by 41,7%.Complete clinical responsse observed in the single infection group number of 66,7%, while in the group of multipleinfections by 50% (p = 1,000). While HPV infection settled with a complete clinical responsse by 16,7% (p = 0,015).There werent clinically relationships between clinical complete responsse with single or multiple HPV infection (p =1,000). There were clinically relationship between persistent HPV infection with complete clinical responsse (p = 0,015).