Abstract
Diffuse large B-cell lymphoma (DLBC) is a kind of tumor with rapid progress and poor prognosis. Therefore, it is necessary to explore new biomarkers or therapeutic targets to assist in diagnosis or treatment. This study is aimed at screening hub genes by weighted gene coexpression network analysis (WGCNA) and exploring the significance of overall survival (OS) in DLBC patients. Statistical data using WGCNA to analyze mRNA expression in DLBC patients came from The Cancer Genome Atlas (TCGA) dataset. After analyzing with clinical information, the biological functions of hub genes were detected. Survival analysis, Cox regression detection, and correlation analysis of the hub genes were carried out. The potential function of the hub gene related to prognosis was predicted by gene set enrichment analysis (GSEA). The results showed that APOE, CTSD, LGALS2, and TMEM176B expression in normal tissues was significantly higher than that in cancer tissues (P < 0.01). Survival analysis showed that patients with high APOE and CTSD were associated with better OS (P < 0.01). APOE and CTSD genes were mainly enriched in the regulation of ROS and oxidative stress. The two hub genes related to the prognosis of DLBC were identified and verified based on WGCNA. Survival analysis showed that the overexpression of APOE and CTSD in DLBC might be beneficial to the prognosis. These findings identified vital pathways and genes that may become new therapeutic targets and contribute to prognostic indicators.
Highlights
Diffuse large B-cell lymphoma (DLBC) is one of the most common types of invasive non-Hodgkin’s lymphoma (NHL), accounting for about 25% of NHL cases [1]
gene set enrichment analysis (GSEA) results showed that Apolipoproteins E (APOE) was mainly enriched in fatty acid metabolism, and the regulation of ROS signaling pathways can regulate the body’s metabolism and the microenvironment of tumor cells
The two prognosis-related genes APOE and Cathepsin D (CTSD) were mainly related to regulating tumor and metabolism-related functions
Summary
Diffuse large B-cell lymphoma (DLBC) is one of the most common types of invasive non-Hodgkin’s lymphoma (NHL), accounting for about 25% of NHL cases [1]. The study of genes and signal pathways regulated during tumorigenesis will contribute to the study of the pathological mechanism of DLBC and guide the therapeutic effect [2, 3]. It usually occurs only outside the central nervous system or is less isolated from the central nervous system [4]. It is very aggressive and progresses rapidly, so early treatment is the critical way to save the lives of DLBC patients. There are no specific biomarkers widely used in the clinic for DLBC, which limits the understanding of the pathogenesis of the disease and the predictive risk factors of disease prognosis
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