Huanglian Jiedu Decoction Exerts Antipyretic Effect by Inhibiting MAPK Signaling Pathway.

Honghong Liu,Xiao Ma,Yanling Zhao,Ying Wei,Shengqi Niu,Lisheng Chen,Sichen Ren,Shizhang Wei,Xiang Liu,Xing Li,Manyi Jing,Haotian Li,Yuling Tong

doi:10.1155/2021/2209574

Abstract

Aim The aim of this study was to explore the antipyretic effect and potential mechanism of Huanglian Jiedu Decoction (HLJDD) on LPS-induced fever in rats. Materials and Methods The fever rat model was established by LPS. Anal temperature of rats was measured every 1 hour after modeling. TNF-α, IL-6, PGE2, and cAMP in rat serum or hypothalamus tissue were detected by ELISA kit. In order to explore the potential active ingredients and mechanism of antipyretic effect of HLJDD, we predicted the underlying antipyretic mechanism by using network pharmacology and then verified its mechanism by Western Blotting. Results The results showed that HLJDD can alleviate LPS-induced fever in rats. The expression levels of TNF-α, IL-6, PGE2, and cAMP in the treatment group were significantly lower than those in the model group. Western Blotting results showed that the protein expression of p-ERK, p-JNK, and p-P38 was significantly inhibited. Conclusion The findings suggest that HLJDD has a good antipyretic effect on LPS-induced fever in rats, which may be closely related to the inhibition of MAPK signaling pathway.

Highlights

Fever is a complex physiological stress response characterized by a regulatory rise in body temperature in response to inflammation or infectious disease [1]
Fever is a controlled increase in body temperature, a hypothalamic-mediated response caused by pathogenic injury or invasion [2, 3]. is reaction promotes the synthesis of endogenous heat-producing factors, such as TNF-α, IL-6, PGE2, and cAMP, which will cause a series of biochemical and physiological changes in the body and eventually lead to elevated body temperature [4,5,6,7]
In order to explore the antipyretic effect of Huanglian Jiedu Decoction (HLJDD) on LPS-induced fever in rats, we detected the expression levels of TNF-α and IL-6 in the serum and PGE2 and cAMP in the hypothalamus tissue of fever rats (Figure 2). e expression of TNF-α, IL-6, PGE2, and cAMP in model group was significantly higher than that in control group (P < 0.01)

Summary

Introduction

Fever is a complex physiological stress response characterized by a regulatory rise in body temperature in response to inflammation or infectious disease [1]. The body maintains a dynamic balance between heat production and heat loss. When this balance is upset, the body temperature becomes abnormal. Fever is a controlled increase in body temperature, a hypothalamic-mediated response caused by pathogenic injury or invasion [2, 3]. Hyperthermia is a treatment method that heats the temperature of a specific part of the body or the whole body to above the normal body temperature, so as to achieve the therapeutic effect. Uncontrolled fever is associated with worse outcomes in patients with sepsis or neuronal damage [11]

Methods

Results

Discussion

Conclusion