Huangkui Capsule Attenuates Lipopolysaccharide-Induced Acute Lung Injury and Macrophage Activation by Suppressing Inflammation and Oxidative Stress in Mice.

Jinfang Deng,Wei Chen,Lei Fang,Xiuru Li,Zhenpeng He,Shuangyi Xu,Ziwen Yu,Ting Qi,Zhengxin Xu,Jian-You Guo

doi:10.1155/2021/6626483

Abstract

Background Huangkui capsule (HKC) comprises the total flavonoid extract of flowers of Abelmoschus manihot (L.) Medicus. This study aimed to explore the effects of HKC on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and LPS-stimulated RAW 264.7 cells. Methods Enzyme-linked immunosorbent assay, histopathology, spectrophotometry, and quantitative real-time polymerase chain reaction were used for the assessments. Statistical analysis was performed using a one-way analysis of variance. Results LPS significantly increased lung inflammation, neutrophil infiltration, and oxidative stress and downregulated lung miR-451 expression. Treatment with HKC dramatically, reduced the total cell count in the bronchoalveolar lavage fluid (BALF), and inhibited myeloperoxidase activity in the lung tissues 24 h after LPS challenge. Histopathological analysis demonstrated that HKC attenuated LPS-induced tissue oedema and neutrophil infiltration in the lung tissues. Additionally, the concentrations of tumour necrosis factor- (TNF-) α and interleukin- (IL-) 6 in BALF and IL-6 in the plasma reduced after HKC administration. Moreover, HKC could enhance glutathione peroxidase and catalase activities and upregulate the expression of miR-451 in the lung tissues. In vitro experiments revealed that HKC inhibited the production of nitric oxide, TNF-α, and IL-6 in LPS-induced RAW 264.7 cells and mouse primary peritoneal macrophages. Additionally, HKC downregulated LPS-induced transcription of TNF-α and IL-6 in RAW 264.7 cells. Conclusions These findings suggest that HKC has anti-inflammatory and antioxidative effects that may protect mice against LPS-induced ALI and macrophage activation.

Highlights

Acute lung injury (ALI), a common lung inflammatory disease, is characterised by tachypnoea, progressive hypoxaemia, and reduced lung compliance
Enzymelinked immunosorbent assay (ELISA) kits for mouse tumour necrosis factor- (TNF-)α and IL-6 were obtained from Biolegend Co. (San Diego, California, USA). e EL-TMB Chromogenic Reagent Kit and 3, 3′, 5, 5′-tetramethylbenzidine (TMB) were purchased from Sangon Biotech Co. (Shanghai, China)
Lung histopathologic changes and inflammation scores were measured to evaluate the protective effect of Huangkui capsule (HKC) on LPS-induced acute lung injury (ALI) in mice

Summary

Introduction

Acute lung injury (ALI), a common lung inflammatory disease, is characterised by tachypnoea, progressive hypoxaemia, and reduced lung compliance. E lung-protective mechanical ventilation is a standard therapeutic method for ALI/ARDS patients. Is study aimed to explore the effects of HKC on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and LPSstimulated RAW 264.7 cells. Treatment with HKC dramatically attenuated the lung wet/dry weight ratio, reduced the total cell count in the bronchoalveolar lavage fluid (BALF), and inhibited myeloperoxidase activity in the lung tissues 24 h after LPS challenge. Histopathological analysis demonstrated that HKC attenuated LPS-induced tissue oedema and neutrophil infiltration in the lung tissues. In vitro experiments revealed that HKC inhibited the production of nitric oxide, TNF-α, and IL-6 in LPS-induced RAW 264.7 cells and mouse primary peritoneal macrophages. HKC downregulated LPS-induced transcription of TNF-α and IL-6 in RAW 264.7 cells. Ese findings suggest that HKC has anti-inflammatory and antioxidative effects that may protect mice against LPS-induced ALI and macrophage activation Conclusions. ese findings suggest that HKC has anti-inflammatory and antioxidative effects that may protect mice against LPS-induced ALI and macrophage activation

Methods

Results

Conclusion