Abstract
Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related mortality worldwide. It is urgently needed to develop potential drugs with good efficacy and low toxicity for HCC treatment. The anti-tumor effect of Traditional Chinese Medicine (TCM) has received increasing attention worldwide. Trametes robiniophila Murr. (Huaier) has been used in TCM for approximately 1,600 years. Clinically, Huaier has satisfactory therapeutic effects in cancer treatment, especially in HCC. However, the mechanisms underlying the anti-cancer effect of Huaier remain ill defined. Herein we have demonstrated that Huaier dramatically inhibited cell proliferation and induced apoptosis in human hepatoma cell line SKHEP-1. Importantly, Huaier restrained the metastatic capability of SKHEP-1 cells. Mechanistically, down-regulation of Lamin B1 and up-regulation of Nephroblastoma overexpressed (NOV) were at least partially responsible for the inhibitory effect of Huaier on the proliferative and invasive capacity of SKHEP-1 cells. Our finding provided new insights into mechanisms of anti-HCC effect of Huaier and suggested a new scientific basis for clinical medication.
Highlights
To explore whether the decreased Lamin B1 and increased NOV induced by Huaier were responsible for the suppression of proliferation and metastasis in SKHEP-1 cells exposed to Huaier, we investigated the effects of blockage of Lamin B1 expression or NOV expression on proliferation and metastasis of SKHEP-1 cells by using RNA
We have demonstrated that suppression of proliferation and metastasis, and induction of apoptosis in SKHEP-1 cells by Huaier were at least partially due to down-regulation of Lamin B1 and up-regulation NOV
We demonstrated that Huaier significantly inhibited the proliferation of human hepatoma cell line SKHEP-1, which was partially due to Huaier-induced cell-cycle arrest in G0/G1 phase (Fig. 1)
Summary
Our result indicated that Huaier aqueous extract significantly inhibited proliferation of SKHEP-1 cells (Fig. 1A). Colony formation assay was performed to evaluate the effect of Huaier aqueous extract on SKHEP-1 cell proliferation. These data revealed that Huaier-induced G0/ G1 phase arrest via p18 pathway partially contributes to inhibition of SKHEP-1 cell proliferation.
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