Https://microbiologyjournal.org/in-vitro-evaluation-of-chitosan-hydroxyapatite-nanocomposite-scaffolds-as-bone-substitutes-with-antibiofilm-properties/

Anuj Nishanth Lipton,S.G.P Vincent,Aifa Fathima

doi:10.22207/jpam.15.3.39

Abstract

An opaque, white chitosan/ Hydroxyapatite nanocomposite was prepared by a simple blend method. Morphology, pore size and dispersion of nano-hydroxyapatite in chitosan matrix were visualized using SEM images. The FTIR and SEM with EDX analysis confirmed the bony apatite layer was formed on the outside of the composite. Porosity measurements and water uptake studies of the nanocomposite were evaluated which revealed the maximum porosity of 80% to 92% in the chitosan: hydroxyapatite nanocomposite at the ratio of 20:80. The results also showed that water absorption ability was inversely proportional to the hydroxyapatite present in the nanocomposite. The porosity of prepared nanocomposite was corresponding to the cancellous bone porosity of 50% to 90% suggesting possible applications in bone transplantation. The nanocomposite exhibited antibacterial activity towards the tested Gram-negative and Gram-positive species of bacteria and reduced the bacterial adhesion in biofilm formation.

Highlights

An opaque, white chitosan/ Hydroxyapatite nanocomposite was prepared by a simple blend method
The results showed that water absorption ability was inversely proportional to the hydroxyapatite present in the nanocomposite
The activities were largely dependent on the presence of Chitosan within the composite, and it was noted that the presence of Hydroxyapatite did not affect the ability to reduce microbial growth

Summary

Introduction

White chitosan/ Hydroxyapatite nanocomposite was prepared by a simple blend method. Maximum porosity was observed in Chitosan: Hydroxyapatite nanocomposite with a ratio of 20:80 which showed 80-92% of porosity (Fig. 1). From the results obtained and as it could be noted from the Fig. 2, the nanocomposite absorbed more water than their own weight, this is the reason the percentage is above 100.0% and the scaffold showed maximum capacity to retain the absorbed water. The maximum water uptake ability was observed in Chitosan/ Hydroxyapatite composite with a ratio of 100/5 which showed

Results

Conclusion