Abstract
Summary The alcaline phosphatase (E.C. 3.1.3.1) associated with the microsome fraction of murine plasmocytomas was studied, using both quantitative and qualitative criteria of analysis. Specific enzyme activity ranged from 35 to 170 Units; normal intestine contained 120 Units and normal liver and spleen 0,2 and 0,6 Units, respectively. Following acrylamide - agarose gel electrophoresis, a multi-zone type migration is obtained. Each of twelve plasmocytomas tested has a distinct pattern. Except for one tumor, this pattern is stable over one year (13–15 transplant generations in vivo). Incubation of the alcaline phosphatase with Vibrio cholerae neuraminidase (E.C. 3.2.1.18) leads to a complete loss of anodic bands and a simultaneous increase in the intensity of a slow band. It is suggested that sialic acid residues attached to the enzyme give rise to the electrophoretic pattern observed. Immunological analyses indicate an antigenic relationship of the electrophoretically different enzymatic forms within a single tumor. Moreover, alcaline phosphatases of all the plamocytomas tested share common antigenic determinants.
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