Abstract
This research aims to investigate the HtrA1 serine protease circulating level of Age-related Macular Degeneration (AMD) patients in Yogyakarta, Indonesia. This study was conducted from January to August 2019 which included 38 AMD patients and 16 Non-AMD patients/controls (two groups). Baseline data and blood sample were collected. ELISA assay was used to measure the HtrA1 serine protease circulating level on both groups. SNP genotyping of rs10490924 was using restriction enzyme digestion. This study used The IBM SPSS® version 24 (Chicago, The USA) to determine the relationship between HtrA1 expression level and AMD incidence. AMD patients had higher HtrA1 serine protease level (35.31) than controls (30.08). However, there is no association found between HtrA1 serine protease level and AMD incidence (p-value>0.05, CI 95 %). However, HtrA1 serine protease did not associate positively to AMD incidence in Yogyakarta samples. Further analysis by grouping AMD patient based on the rs10490924 genotype show no statistical correlation between HTRA1 to the incidence of AMD. This result might be due to the lack of samples in the study groups. Future studies with larger number of samples are advised to better see the association between Htra1 serine protease level and AMD incidence.
Highlights
Age-related Macular Degeneration (AMD) is a degenerative disease that compromise the photoreceptor cells, especially at the macula, a specific area of the retina [1]
170 × 106 people are suffering impairment acuity or blindness caused by AMD in the world, and among 11 × 106 of it is in the United States
Data normalization was done to balance both arms by reducing the number of samples from AMD group into only 16 samples using SPSS software
Summary
Age-related Macular Degeneration (AMD) is a degenerative disease that compromise the photoreceptor cells, especially at the macula, a specific area of the retina [1]. 170 × 106 people are suffering impairment acuity or blindness caused by AMD in the world, and among 11 × 106 of it is in the United States The prevalence of this disease was increases along the growth of the elderly population, especially in people above 50 yr old Caucasian. ARMS2 protein deficiency alone is not induce the disease, those common risk alleles alter ARMS2 but not HTRA1 expression [4] These polymorphisms might influence in different biological pathways of AMD pathogenesis. Based on the epidemiological data, Genetic factors including gene polymorphisms of age-related maculopathy susceptibility 2 (ARMS2), high temperature requirement protease A1 (HTRA1), and complement factor H (CFH) strongly associated to the onset of AMD. This research aims to investigate the HTRA1 circulating level on Age-related Macular Degeneration (AMD) according to the rs10490924 genotype in Yogyakarta, Indonesia
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