Abstract
BackgroundWe evaluated whether heat shock protein HSP70 plays a protective role in the embryos of Kunming mice subjected to chronic unpredictable mild stress.MethodsFemale mice were stimulated for 4 weeks with nine stressors and then divided into mild, moderate and severe stress groups. Superovulation was induced with a gonadotropin preparation (PMSG/HCG) and HSP70 expression in 2-cell embryos and day 4 embryos was detected by immunofluorescence (IF) and real-time polymerase chain reaction (RT-PCR).ResultsIn the mild stress group, ovarian response and oocyte development potential were similar to those of the control group, while the HSP70 mRNA levels of the embryos were significantly higher (P < 0.05). In the severe stress group, ovarian response and oocyte development potential decreased compared with the control group (P < 0.05), while the HSP70 mRNA levels were similar. The results of the moderate stress group were intermediate among the three groups. Furthermore, HSP70 mRNA levels of the embryos were shown to be positively associated with parameters of oocyte and embryo development potential (P < 0.05).ConclusionsHSP70 overexpression may play a protective role in the embryos of the mild or moderate stress mice stimulated by chronic unpredictable mild stress.
Highlights
We evaluated whether heat shock protein HSP70 plays a protective role in the embryos of Kunming mice subjected to chronic unpredictable mild stress
We found that after the administration of pregnant mares’ serum gonadotropin (PMSG) and human chorionic gonadotropin to induce superovulation, the numbers of oocytes and embryos obtained from the mice decreased under Chronic unpredictable mild stress (CUMS) conditions
Division of experimental group According to the data of the Open field test (OFT) and the sucrose consumption test, we found that the time spent in center, the distance moved, rearing count and defecation of the OFT and the sucrose consumption were different between the experimental group and the control group
Summary
We evaluated whether heat shock protein HSP70 plays a protective role in the embryos of Kunming mice subjected to chronic unpredictable mild stress. Stress is a complex state of threatened homeostasis, which mobilizes a composite spectrum of nervous, endocrine, and immune system responses to restore and maintain homeostasis. Animal models cannot replicate human psychopathology in every detail, they offer the possibility of evaluating the main effects and interactions in a controlled, prospective manner. Chronic unpredictable mild stress (CUMS) is one of the behavioral models used to simulate human depression in some respects, such as loss of normal aggressiveness [19].
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