HSP70 and HSP90 expression in leucocytes after exercise in moderately trained humans.

Abstract

In this study, we examined expression of heat shock proteins (HSP) 70 and 90 in human leucocytes after moderate-to-heavy exercise. We also compared baseline levels of HSP70 and HSP90 in trained (TR) and untrained (UT) subjects. Eleven TR subjects ran on a treadmill for 1 h at 70% of maximal oxygen consumption. The HSP levels were measured prior to exercise and 15 and 24 h after exercise. Baseline HSP levels were also measured in eight UT controls. Fifteen hours and 24 h after exercise, TR subjects showed no significant increases in HSP70 (P > 0.05). The HSP90 levels also did not change (P > 0.05). Baseline HSP70 levels in TR subjects were lower than in UT subjects (2.04 +/- 0.51 ng vs. 4.52 +/- 0.95 ng, P < 0.05), while HSP90 levels were similar in TR and UT subjects. We conclude that exercise at an intensity that is within normal limits for a moderately trained individual is not a sufficient stimulus of HSP70 production in leucocytes. We also conclude that blunted levels of baseline HSP70 expression in TR subjects might be a chronic adaptation to training.