Abstract
HSP47, a collagen-specific molecular chaperone, interacts with unfolded and folded procollagens. Binding of chicken HSP47 to native bovine type I collagen was studied by fluorescence quenching and cooperative binding with a collagen concentration at half saturation (K(half)) of 1.4 x 10(-7) m, and a Hill coefficient of 4.3 was observed. Similar results are observed for the binding of mouse HSP47 recombinantly expressed in Escherichia coli. Chicken HSP47 binds equally well to native type II and type III procollagen without the carboxyl-terminal propeptide (pN type III collagen), but binding to triple helical collagen-like peptides is much weaker. Weak binding occurred to both hydroxylated and nonhydroxylated collagen-like peptides, and a significant chain length dependence was observed. Binding of HSP47 to native type I collagen had no effect on the thermal stability of the triple helix. Refolding of type I collagen in the presence of HSP47 showed minor changes, but these are probably not biologically significant. Binding of HSP47 to bovine pN type III collagen has only minor effects on the thermal stability of the triple helix and does not influence the refolding kinetics of the triple helix.
Highlights
HSP47 (47-kDa heat shock protein) is a rough endoplasmic reticulum protein believed to function as a collagen-specific molecular chaperone [1,2,3]
We explore the effect of HSP47 on the thermal stability and refolding of type I and pN type III collagen
Chicken HSP47 was extracted and purified from chick embryos, and mouse HSP47 was expressed in Escherichia coli
Summary
HSP47 (47-kDa heat shock protein) is a rough endoplasmic reticulum protein believed to function as a collagen-specific molecular chaperone [1,2,3]. Binding of chicken HSP47 to native bovine type I collagen was studied by fluorescence quenching and cooperative binding with a collagen concentration at half saturation (Khalf) of 1.4 ؋ 10؊7 M, and a Hill coefficient of 4.3 was observed. Weak binding occurred to both hydroxylated and nonhydroxylated collagen-like peptides, and a significant chain length dependence was observed.
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