HSCCC‐MS Study of Flavonoids in the Extracts from the Seeds of Oroxylum indicum

Abstract

In this study, two analytical high‐speed countercurrent chromatography (HSCCC) instruments, interfaced directly with electrospray ionization and atmospheric pressure chemical ionization mass spectrometry, were explored for the separation of a mixture of three standard flavonoids. Hexane–ethyl acetate–methanol–water solvent systems with different ratios were chosen and optimized for the best separation. On‐line HSCCC‐MS was successfully used in the identification of flavonoids from an ethyl acetate extract of the seeds of Oroxylum indicum.