Hsa_circ_0001982 promotes the proliferation, invasion, and multidrug resistance of osteosarcoma cells.

Abstract

BackgroundOsteosarcoma (OS) is the most common bone cancer mostly seen in people aged 10–25 years. This research aims to clarify the function of hsa_circ_0001982 in osteosarcoma (OS) and its effect on drug resistance, preliminarily exploring its mechanism.MethodsThe expression of hsa_circ_0001982 and miR‐143 in OS clinical tissues and cells was detected by real‐time fluorescence quantitative polymerase chain reaction (qRT‐PCR), MTT, colony formation assay, and transwell assay assessed cell proliferation, colony formation, migration, and invasion, respectively. The targeted relationship of hsa_circ_0001982 and miR‐143 was verified by a dual‐luciferase reporter assay.ResultThe expression of hsa_circ_0001982 was significantly increased in OS tissues and cells (MG63), as in well as chemoresistant OS tissues and cells (MG63/Dox). Overexpression of hsa_circ_0001982 promoted proliferation, colony formation, migration, invasion, and multidrug resistance in MG63 cells. By contrast, knockdown of hsa_circ_0001982 markedly reduced the resistance of MG63/Dox cells to doxorubicin (IC50 evidently reduced). Bioinformatic prediction showed that miR‐143 was a target miRNA of hsa_circ_0001982, and a dual‐luciferase reporter assay proved this. Further experiments revealed that miR‐143 expression was notably downregulated in OS tissues, chemoresistant OS tissues, and MG63/Dox cells. Moreover, miR‐143 was negatively correlated with hsa_circ_0001982 in OS cells and tissues.ConclusionThe regulation of malignant behaviors such as proliferation, invasion, migration, and multidrug resistance of OS cells by hsa_circ_0001982 may be achieved by targeting miR‐143. Moreover, hsa_circ_0001982 is a potential target for early diagnosis and targeted therapy of OS.