HRRA1 and type VI collagen are upregulated by single impact load in a novel model of early oa in human cartilage

Abstract

Event Abstract Back to Event HRRA1 and type VI collagen are upregulated by single impact load in a novel model of early oa in human cartilage FMD Henson1*, P Hernandez1, J Wardale1 and N Rushton1 1 Orthopaedic Research Unit, Department of Surgery, United Kingdom Introduction: HTRA1, a serine protease, has been shown to be upregulated in osteoarthritis (OA). It has been proposed that HTRA1 protein is upregulated as an initial event in OA which leads to breakdown of type VI collagen, an important pericellular matrix component. In order to investigate the relationship of HTRA1 and type VI collagen in the early events of human OA this work aims to i) characterise a single impact load (SIL) model of cartilage damage in human tissue and ii) describe the immunolocalisation of HTRA1 and type VI collagen in this model.{BR}Materials and Methods: 7mm full thickness cartilage discs were obtained from normal hips obtained from patients with femoral neck fractures (n=4). Cartilage was SIL with 250g from various heights, cultured for up to 10 days and processed for routine histology. Immunohistochemistry was performed using anti-HTRA1 and anti-type VI antibodies.{BR}Results: 2.5cm was chosen as the height from which SIL of 250g should be applied to mimic tissue damage seen in OA as it causes characteristic tissue damage (surface lamination, fissure formation, loss of toluidine blue and apoptosis at the surface of the cartilage). HTRA1 immunoreactivity was not present in normal cartilage at t = 0 days and was seen in the surface zone of control cartilage at 4d of culture onwards. In SIL cartilage HTRA1 was detected in the surface zone at day 1 and throughout the cartilage by day 10. Type VI collagen was detected in the surface and mid zone in control cartilage and strongly throughout the cartilage in SIL samples. HTRA1 and type VI collagen were shown to co-localise in a number of chondrocytes.{BR}Discussion: This study describes a SIL model of early OA in human cartilage. In this model it is demonstrated that HTRA1 and type VI collagen immunoreactivity is increased after SIL and culture and that the HTRA1 and type VI collagen can be co-localised. These results challenge previous findings by other workers in murine models and suggest an alternative hypothesis for the role of HTRA1 in OA. Keywords: Bones, Bone Research Conference: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society, Cambridge, United Kingdom, 27 Jun - 29 Jun, 2011. Presentation Type: Poster Topic: Abstracts Citation: Henson F, Hernandez P, Wardale J and Rushton N (2011). HRRA1 and type VI collagen are upregulated by single impact load in a novel model of early oa in human cartilage. Front. Endocrinol. Conference Abstract: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society. doi: 10.3389/conf.fendo.2011.02.00027 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2011; Published Online: 30 Sep 2011. * Correspondence: Dr. FMD Henson, Orthopaedic Research Unit, Department of Surgery, Cambridge, United Kingdom, fmdh1@cam.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers FMD Henson P Hernandez J Wardale N Rushton Google FMD Henson P Hernandez J Wardale N Rushton Google Scholar FMD Henson P Hernandez J Wardale N Rushton PubMed FMD Henson P Hernandez J Wardale N Rushton Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.