Abstract
A variety of mediators are involved in the pathophysiology of a number of clinical conditions. Among them histamine-releasing factors (HRF) act as secretagogue for basophils and mast cells to cause cell degranulation and histamine release. Studies on the kinetics of HRF production by activated mononuclear cells (MNC) have suggested an active synthesis of this cytokine. Using a [35S]-metabolic cell labeling method, we first analyzed the capacity of MNC from patients with allergic rhinitis to ragweed to actively synthesize HRF. We then found that stimulation of MNC with allergen promoted a synthesis of large quantities of proteins, including a protein of about 30 kDa. Secondly, we developed a method for the enrichment of HRF molecules from crude supernatants of ragweed-stimulated MNC using ion exchange and gel filtration chromatography. We observed that the yield of HRF activity is seen just before the chymotrypsinogen marker. The estimated molecular weight was 30-35 kDa.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
