Abstract
High resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy is increasingly used for profiling of breast cancer tissue, delivering quantitative information for approximately 40 metabolites. One unique advantage of the method is that it can be used to analyse intact tissue, thereby requiring only minimal sample preparation. Importantly, since the method is non-destructive, it allows further investigations of the same specimen using for instance transcriptomics. Here, we discuss technical aspects critical for a successful analysis—including sample handling, measurement conditions, pulse sequences for one- and two dimensional analysis, and quantification methods—and summarize available studies, with a focus on significant associations of metabolite levels with clinically relevant parameters.
Highlights
The improved understanding of breast cancer has been supported by the development of omics-based technologies
In another study of the same authors, where tumour tissue specimens from 89 breast cancer patients who received neoadjuvant chemotherapy were analysed, survivors showed a significant decrease in the levels of glycine, choline, phosphocholine and glycerophosphocholine and an increase in glucose, post-treatment compared to pre-treatment; whereas, non-survivors displayed an increased level of lactate after treatment [37]
This review provides an overview of analytical aspects related to the use of quantitative High resolution magic-angle spinning (HR-MAS)
Summary
The improved understanding of breast cancer has been supported by the development of omics-based technologies. As. In addition, the risk of partial extraction of certain metabolites, as for instance reported for choline [19], already mentioned, HR-MAS NMR allows metabolite quantification directly from intact tissue is minimized. The non-destructive nature of the method allows the analysis of metabolite levels specimens, which abolishes the need for an extraction step and thereby avoids one potential source in tissue to be combined with other analytical techniques based on the same tissue specimen, which of poor reproducibility. We summarize findings aspects, including measurement conditions, pulse sequences used for 1D and 2D NMR, based on quantitative HR-MAS NMR with regard to significant associations with clinically relevant quantification methods and the numbers and identities of the reported metabolites. Summarize findings based on quantitative HR-MAS NMR with regard to significant associations with clinically relevant factors in breast cancer
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