HPV16 infection and p53 codon 72 polymorphism in cervical cancer and precursor lesions in Hubei, China.

Abstract

e15582 Background: The aim of this study was to investigate the prevalence of HPV16 infection and distribution of p53 codon72 polymorphism with cervical cancer and precursor lesions and to correlate these to cervical cancer risk in Chinese population in Hubei, China. Methods: A total of 104 patients with cervical squamous-cell carcinoma (SCC) and 52 patients with cervical intraepithelial neoplasia (CIN) II-III and 88 Patients with CIN I were obtained from women undergoing biopsy or surgery. HPV16 and p53 codon72 polymorphism were examined by PCR. Results: The prevalence of HPV16 was 78.5% in the SCC group, 44.2% in the CINII- III group and 16.7% in the CIN I group. There was significantly association between HPV16 positivity and cervical neoplasia progression (Spearman R=0.550, P=0.001). The percentages of Arg/Arg, Pro/Arg and Pro/Pro genotypes in the SCC group were 38.5%, 44.2%, 17.3%, and in CIN I group were 52.4%, 40.5%, 7.1%, respectively. The RR (risk ratio) of Pro/Pro genotype was 3.300 (95% CI=1.192-9.136) compared with Arg/Arg genotype. The frequencies of p53 Arg/Arg genotype in SCC group were not statistically different from those in the CIN I and CINII- III group. The distributions of p53 codon72 genotype Arg/Arg, Arg/Pro, Pro/Pro were 32.8%, 57.1%, 14.2% in the HPV16 infected SCC group; 27.1%, 47.8%, 30.4% in the HPV16 infected CINII- III group; and 28.6%, 57.1%, 17.3% in the HPV16 infected CIN I group respectively. The distribution of p53 genotypes was not significantly different from each other in the three groups (P=0.517). The frequency of p53 Arg/Arg genotype in HPV16 negative SCC group (50%) was not statistically different from that in the HPV16 positive SCC group (P=0.210). Conclusions: The p53 codon72 Pro/Pro genotype was one of the highest risk factors of precancerous cervical lesion in Chinese population in Hubei areas.