HPV Strain Predicts Severity of Juvenile-Onset Recurrent Respiratory Papillomatosis with Implications for Disease Screening.

Abstract

Simple SummaryAlthough it is known that low-risk HPV causes juvenile-onset recurrent respiratory papillomatosis (JoRRP), viral factors such as HPV strain are not routinely determined as part of clinical care. Age at disease onset and HPV strain are two commonly discussed potential predictors of increased disease severity; however, research has been inconclusive and has not yet impacted clinical diagnosis or management of patients with JoRRP. In this prospective study, we found that HPV11+ disease was associated with earlier age of onset, need for surgical intervention, and higher risk of progression to malignancy. These results support that determination of HPV6+ vs. 11+ disease status represents a predictive screening biomarker for disease severity and progression in JoRRP patients. To this end, we provide proof of concept that demonstrates the utility and feasibility of HPV genotyping using RNA-ISH for screening of HPV11+ disease.Juvenile-onset recurrent respiratory papillomatosis (JoRRP) is the most common benign neoplasm of the larynx in children, presenting with significant variation in clinical course and potential for progression to malignancy. Since JoRRP is driven by human papillomavirus (HPV), we evaluated viral factors in a prospective cohort to identify predictive factors of disease severity. Twenty children with JoRRP undergoing routine debridement of papillomas were recruited and followed for ≥1 year. Demographical features, clinical severity scores, and surgeries over time were tabulated. Biopsies were used to establish a tissue bank and primary cell cultures for HPV6 vs. HPV11 genotyping and evaluation of viral gene expression. We found that patients with HPV11+ disease had an earlier age at disease onset, higher frequency of surgeries, increased number of lifetime surgeries, and were more likely to progress to malignancy. However, the amplitude of viral E6/E7 gene expression did not account for increased disease severity in HPV11+ patients. Determination of HPV strain is not routinely performed in the standard of care for JoRRP patients; we demonstrate the utility and feasibility of HPV genotyping using RNA-ISH for screening of HPV11+ disease as a biomarker for disease severity and progression in JoRRP patients.