Abstract
A sensitive and reliable high-performance thin layer chromatographic method has been developed for quantitation of camptothecin in the dry stem powder of Nothapodytes foetida (Wight) Sleumer. A methanolic extract of the dry powder was chromatographed on silica gel 60F 254 plates with toluene-acetonitrile-glacial acetic acid, 6.5 + 3.5 + 0.1 ( v / v ), as mobile phase. Detection and quantitation were performed by densitometric scanning, in fluorescence mode at λ = 370 nm, by use of a mercury lamp. The accuracy of the method was checked by determination of recovery, using the standard-addition method. Recovery was 99.49%. The average camptothecin content of the powder was 0.059%. The method is rapid, simple, and precise.
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